a study of polymorphism in exon 6 of calpastatin gene through pcr- sscp method and its association with carcass traits in lori-bakhtiari and zel-atabay sheep

calpastatin has been introduced as an effective candidate gene as regards growth efficiency and meat quality traits. throughout the present study, blood sampling as well as carcass trait measurements were performed on 74 lori-bakhtiari sheep and on 40 zel-atabay cross-breds from industrial slaughterhouses of shahrekord and gorgan cities, respectively. following dna extaction, polymerase chain reactions were performed for amplification of a 254 bp fragment containing the entire exon 6 of calpastatin gene. sscp method along with silver staining were employed for genotyping of pcr products. fourteen genotypic patterns namely: aa, bb, ab, ac, ad, be, af, ag, ah, ai, jj, aj, bk, and al were observed with frequencies of 0.035, 0.123, 0.105, 0.114, 0.009, 0.053. 0.316, 0.027, 0.017, 0.017, 0.009, 0.105, 0.017, and 0.053, respectively for the two breeds. the calpastatin gene significantly affected live weight before slaughter, fat-tail weight(p